Here are your MiTeGen Hottest Tweets in SBDD with exciting software developments such as newly...
MiTeGen Hottest Tweets in SBDD (02/24)
Shots fired: When is it time-resolved? What's the resolution? China to break into TEM? JEOL = Concorde cockpit? Here are your MiTeGen Hottest Tweets in SBDD including some truly remarkable sample prep stories:
Our cryostat has arrived, looking forward to perfectly vitrified ice and no more broken grids! pic.twitter.com/SNcuDccedv
— Coleman Lab (@1nsaneNmembrane) February 8, 2024
Tough drug targets
Excited to share our article on nicotinic receptor modulation, led by talented PhD student Sean Burke. Detailed insights through single channel analysis with Steve Sine's lab, and MD simulations with Marco Cecchini's group. https://t.co/aer5VU3qaW
— Ryan Hibbs (@RyanHibbs10) February 20, 2024
Structures and SAR of B0AT1 (SLC6A19) in complex with nM inhibitors bound to the S2 site!https://t.co/QQyp91qSxr
— David B Sauer (@DavidBSauer) February 20, 2024
Symmetric transthyretin (55 kDa) has been crystallized hundreds of times - why look at it by cryoEM? Good thing @BenjaminBsnt did! Asymmetry, distinct binding pockets, multiple conformations - explains negative cooperativity & amyloidogenic pathway: https://t.co/bOpbcZYV61 pic.twitter.com/xE9PM9SrSa
— Lander Lab (@LanderLab) January 24, 2024
The weird and wonderful
When you do #cryoem but you're trained in crystallography pic.twitter.com/abIlhnAsen
— Christopher P. Jones (@cpricejones) December 20, 2023
Sample preparation
Our cryostat has arrived, looking forward to perfectly vitrified ice and no more broken grids! pic.twitter.com/SNcuDccedv
— Coleman Lab (@1nsaneNmembrane) February 8, 2024
Very cool strategy to overcome AWI problems! The authors showed that the addition of 16-30 kDa LEA proteins did not have a significant effect on the SNR, and the proteins of interest could be investigated at 10-fold lower concentrations than what would be needed with detergents👏 https://t.co/JE1hpESq8U
— Ieva Drulyte (@IevaDrulyte) February 10, 2024
Although the grid graveyard @MPIbp would've easily paid for a Tesla, it bugs me a bit that there's no mention at all of payroll costs for (scientific) staff spending time on cleaning, inspecting and sorting grids by grid type. https://t.co/m2gWHCTMe7
— Sonja Welsch (@sonjawelsch) January 25, 2024
A new automated platform for cryo-EM/ET grids preparation.
— Wojtek Galej (@WojtekGalej) January 22, 2024
Great engineering by @PappTeam and collaborative @embl effort to validate the approach. https://t.co/JuNB7A6CuG pic.twitter.com/xomGsQED7v
Magnetic Isolation and Concentration (MagIC)-cryo-EM is a new method to enable direct structural analysis of targets captured on nanomagnetic beads, which requires only 0.0005 mg/mL of targets in 100 µL crude solution to make 10 cryo-EM grids. I will explain the magic behind it! pic.twitter.com/PkEwu8Qtbg
— Yasuhiro Arimura (@ArmrYshr) January 22, 2024
From the initial buffer additives to the final beam exposure, MiTeGen supports your cryo-EM sample prep needs every step of the way! Grids, grid boxes, pucks, storage & shipping systems, reagents and more! #MiTeGen #cryoem #structuralscience Learn more -> https://t.co/b7dQMjhpR0 pic.twitter.com/Q1UCxDl0LJ
— MiTeGen (@MiTeGen) February 26, 2024
Better imaging
Further evidence of high-resolution capabilities at 100 keV with a side-entry cryo holder using the @GatanMicroscopy Alpine detector. It even performed better than the K3 on a small sample - Great collab with colleagues in Verba lab @UCSF https://t.co/Tzku4SZRbP pic.twitter.com/cLou7UIUXX
— Lander Lab (@LanderLab) February 20, 2024
Wow! Seriously! Wow!https://t.co/TrGRuBNoGX
— Matthew Belousoff (@bluesocks81) February 16, 2024
What company are folks using for cryoem maintenance other than paying directly to thermo?
— Tamir Gonen 🎗️ (@gonenlab) February 3, 2024
China unveiled its first domestically produced transmission electron microscope (TEM), the TH-F120, on Saturday, the Science and Technology Daily reported. The groundbreaking development marks a major breakthrough in core technologies and manufacturing capabilities of the… pic.twitter.com/3nloRFMvXn
— Global Times (@globaltimesnews) January 21, 2024
Didn't think I would ever post ApoF, but here we go. First couple hours of test data collection on our new Tundra. How easy was that! And the best part: it's not hidden in a basement, but on the 3rd floor, one flight of stairs away from the office :D pic.twitter.com/qF4EpOBqhP
— Moritz Hunkeler (@moritzhunkeler) January 18, 2024
How many scientists does it take to install Yong's cryo-em monitor pic.twitter.com/O81zJffyNW
— Yong Xiong Lab (@Xiongosome) December 21, 2023
Processing and IT
I don't want to beat our own drum here, but I just wanted to say that #ModelAngelo by @jamaliki1998 is a fantastic program that has yet again completely blown me away today. 🥳
— Sjors Scheres (@SjorsScheres) February 9, 2024
Two web-based tools are introduced to empower RELION users to analyse data efficiently and make informed decisions regarding data processing at multiple steps along the pipeline @IUCr #CryoElectronMicroscopy #RELION #IceThicknessEstimation https://t.co/KZXPZAnixR pic.twitter.com/ivCJRykZsd
— Structural Biology (@ActaCrystD) February 15, 2024
(realising only now that I never properly announced ISOLDE 1.7…)
— Tristan Croll (@CrollTristan) January 26, 2024
I just uploaded a bugfix 1.7.1 release, which should fix the traceback a few users reported when opening MTZ files containing only calculated amplitudes and phases. Missed it because I never do that myself…
The negative stain work here is beautiful. The #cryoEM is overstated: yet another completely unrealistic resolution estimate. Sometimes it feels like the field is reversing... 😬 https://t.co/eBjoRICIad
— Sjors Scheres (@SjorsScheres) January 24, 2024
Thoughts and opinions
I agree. 10-15 seconds is hardly time resolved, they are still equilibrium endpoints....
— Matthew Belousoff (@bluesocks81) February 22, 2024
Biotech is going to have its ChatGPT moment sometime in the next 5 years. Figuring out the human body is quickly being turned into a compute problem AND the expense of that compute is falling faster than Moore's law (chart and text snippet from @Noahpinion article yesterday -… pic.twitter.com/8i0N8LPUr4
— Ben Buchanan (@01Core_Ben) February 18, 2024
— Israel Fernandez (@IsraelF96135088) January 12, 2024
Despite all the good fun that may ensue, what makes me a little sad about messages like the one that triggered the tweet below is this: Do these people have nobody in their lab/department/university that they can ask basic questions? Are they left completely alone? https://t.co/LyByVKo9VP
— Basil Greber (@BJ_Greber) January 9, 2024
Biotech news and drug discovery technologies
NEW: Regeneron, Vertex joined the $100B Club for the 1st time this earnings season
— Andrew Dunn (@AndrewE_Dunn) February 20, 2024
The two now rank among the 15 or so drugmakers valued >$100 billion$VRTX and $REGN are worth more than legacy co's like $GSK and $BMY — here's what that shake-up means: https://t.co/Owyksz5kkt
Wonder what that secretive longevity company funded w/ $3B from @JeffBezos and @yurimilner has been working on?
— Alex Colville (@AlexJColville) February 20, 2024
SCOOP: @altos_labs latest public program is an oligonucleotide inhibiting the retrotransposon LINE-1 to treat progeria. pic.twitter.com/ynsQL2sT8X
List of AI based Biotechs raised investments
— Paras Sharma (@paras_biotech) February 19, 2024
(74 companies)
**Most raised >$100M - wonder how many have assets in the clinic with poc in patients
Source: Guggenheim pic.twitter.com/v1FVfn9Sr2
The climate around healthcare VC investing definitely has its challenges, but I'm having trouble squaring the rather negative sentiment around LP cautiousness towards biotech in this well-written @statnews piece with the recent VC fundraising data from SVB...
— Bruce Booth (@LifeSciVC) January 25, 2024
SVB: "Fundraising… https://t.co/ZzWDpinNOx pic.twitter.com/gvOpkbLaiQ
Ozemphoria
With 124 drugs in development, over 35% drugs are GIP/GLP glucagon receptor agonists and 46% are orals$XBI $LLY $NVO pic.twitter.com/GOpkG1kwW4
— Kazz (@KazzBio) February 22, 2024
Wowsers...Mounjaro sales were $2.2bn in Q4 alone $LLY
— Madeleine Armstrong (@ByMadeleineA) February 6, 2024
First clinical results for the @Amgen GLP-1 drug for weight loss, which in contrast to others is a dual receptor that blocks GIPhttps://t.co/gDHkLQiv8O @NatMetabolism pic.twitter.com/nkzhksr8KT
— Eric Topol (@EricTopol) February 5, 2024
This is huge! I could care less about Wegovy, Catalent is the largest #AAV #GeneTherapy CDMO in the world and the only one with commercial experience. Lots of Qs for existing clinical clients about implications, timelines, pricing, staffing, etc 🤯https://t.co/eD6mxQeay6
— Dr. Nicole Paulk (@Nicole_Paulk) February 5, 2024
About MiTeGen
MiTeGen, founded in 2004 by Cornell University Professor Robert Thorne, is a leading provider of innovative technologies for cryo-EM, crystallography, and other techniques used for probing small molecule and biomolecular structure and function. Our mission is to support researchers in achieving the best possible results throughout the entire process, from sample preparation to data collection.
With a strong emphasis on collaboration, we actively work with academic, government, and industrial researchers in over 40 countries to develop and deliver new tools and methods. Our products have gained recognition and trust within the scientific community, as they have been cited in over 4,500 published research articles. By leveraging concepts and technologies from physics and related fields, we tackle practical challenges faced by our friends and collaborators in the life sciences and other disciplines.
Driven by a passion for cryo cooling, we would like to showcase three of our innovative cryo-EM solutions:
Manual Plunge Cooler and Precision Ethane Cryostat:
Our Manual Plunge Cooler and Precision Cryostat are specifically designed to streamline and optimize your cryo-EM sample vitrification workflow. These systems consist of three components that can be obtained individually or as a complete package, ensuring efficient and successful sample preparation.
Cryo-EM Starter Kits:
Our Cryo-EM starter kits are curated to equip your laboratory with all the necessary tools conveniently packaged together. These kits are ideal for labs aiming to perform basic grid and sample preparation for cryo-EM within their own facility, even on a reduced budget. The kit includes components essential for cryo-EM sample preparation, as well as sample storage and transport solutions.
Cryo-EM AutoGrid Cassette Puck:
Our latest addition, the Cryo-EM AutoGrid Cassette Storage and Shipping puck, builds upon the success of our 2nd generation Cryo-EM Puck. This innovative solution allows users to screen samples on a instrument and seamlessly ship the same AutoGrid cassette puck to a collection facility. It also serves as a convenient storage option for facilities, enabling them to store their cassettes in the puck canes designed to fit into cassette loading stations.
These cryo-EM solutions exemplify our dedication to enhancing cryo cooling capabilities and simplifying workflows in the field of cryo-EM. MiTeGen continues to push the boundaries of innovation, enabling researchers to advance their structural biology studies with greater ease and efficiency.
Contact us at MiTeGen today to arrange a no obligation consultation to learn how we can help you with your research.
