Here are your MiTeGen Hottest Tweets in SBDD: While the AlphaFold 3 release made a big splash with...
MiTeGen Hottest Tweets in SBDD (04/24)
Here are your MiTeGen Hottest Tweets in SBDD, featuring heated discussions about the Inflation Reduction Act, controversially dubbed the 'Innovation Reduction Act' by critics, while the cryo-EM field continues to produce stunning structures and innovative methods.
Tough drug targets
An exciting time in Solute Carrier research. Here are some of the areas where SLC structural biology is heading and where future opportunities for new discoveries and therapeutic interventions await. https://t.co/JHeCx7weEt https://t.co/UVm6apASDk @BiochemOxford @KavliOxford
— Simon Newstead (@inwardopen) April 18, 2024
Congrats to @KYoojoong @rgumpper @RothLabUNC
— BryanRoth (@zenbrainest) April 10, 2024
Bitter taste receptor activation by cholesterol and an intracellular tastanthttps://t.co/NgIl2rk01V
"Here, we present a #cryoEM analysis of a trimeric complex composed of the nucleosome core particle (NCP), RCC1, and Ran."
— Biochemistry (@BiochemistryACS) March 29, 2024
Huang, Rubinstein and Kay discover dynamic and transient interactions enabled by a nucleosome hub. https://t.co/buutmL3UUN@UofT@SickKidsNews pic.twitter.com/i2WuYVkX1A
My take on high-resolution #cryoEM of small complexes has just been published in @Structure_CP. In my review, I first introduce how technical developments have enabled #cryoEM structure determination of ever smaller targets at better and better resolutions. pic.twitter.com/dc99YnXXBM
— Basil Greber (@BJ_Greber) April 1, 2024
Structural bases of inhibitory mechanism of CaV1.2 channel inhibitors | Nat Communhttps://t.co/aUieZPQTNl pic.twitter.com/tahtnNYo9C
— Membrane Protein PDB (@MemProtPDB) April 1, 2024
The weird and wonderful
Alright everybody, what will undoubtedly forever be the lab's strangest discovery is now out in Nature. It's a protein that evolved to self-assemble into a Sierpinski fractal. https://t.co/zV5CDzbaGm 1/n
— Georg Hochberg (@KaHochberg) April 10, 2024
😂🤣😎🔬 pic.twitter.com/Y3EfgC2aWp
— Arvind Kumar (@ArvindQuantum) April 10, 2024
Years after the idea and preliminary experiments, Claire Coupland's amazing structure of the endogenous vacuolar- or vesicular-ATPase in its native vesicle is now in bioRxiv
— John Rubinstein (@RubinsteinJohn) April 2, 2024
"High resolution cryo-EM of V-ATPase in native synaptic vesicles"https://t.co/fuA8UOBJCY pic.twitter.com/m9NVvvturH
Sample preparation
Setting up a new lab or know someone who is? Contact us for special pricing & bundles on lab equipment & supplies to get the most out of those precious start-up funds. Let us be your partner! https://t.co/KJwFnxz1QS #crystallography #cryoEM #beamline #structuralbiology #newpi pic.twitter.com/xRlnSG3LiD
— MiTeGen (@MiTeGen) April 8, 2024
Our preprint by @chenyaoli2013 and @KWillegems describes a simple and rapid method for purifying RyRs on the bench using nanobodies generated in @jansteyaert lab. RyRs can be purified in lipid nanodiscs or reconstituted into liposomes for #cryoem. https://t.co/QhXK0zK9mg pic.twitter.com/1eN4biNVgC
— Rouslan Efremov (@RouslanEfremov) April 5, 2024
Better imaging
Lots of updates in #SPACEtomo by @EisFabian. New target inspection/selection GUI 🖥️🗺️🥳and a video tutorial: https://t.co/b439IpoqyM
— Rado Danev (@RadoDanev) April 15, 2024
Hallelujah! He has risen!
— Jake Smith (@crying_EM) April 1, 2024
(jk the autoloader is broken) pic.twitter.com/rCwSv2qOdQ
High-res In-situ Structures of Mammalian Respiratory Supercomplexes in Reaction within Native Mitochondria https://t.co/YZZ2njfWd4
— Jack Zhang Lab (@zhang_lab) April 3, 2024
Check out my lab's first work on membrane proteins!
Yes, we knew nothing about detergent😜, so we directly imaged proteins within native membrane! pic.twitter.com/BHBo0rZn07
Laser breadboards are back on the laser phase-contrast microscope pic.twitter.com/dpglxK5aml
— Mueller Lab (@TheMuellerLab) March 19, 2024
Processing and IT
Here is a AlphaFold/ColabFold tutorial we have recently presented - from basic to more advanced usage, enforcing alternative conformations, modeling interactions, and analysis with ChimeraX and PICKLUSTER. Maybe you find it helpful 🎓 ! Commenting enabled in the doc so feel free… pic.twitter.com/FB1xyT5K2O
— Jan Kosinski (@jankosinski) April 12, 2024
Have you tried #Blush in #relion5? That might help. 🤗
— Sjors Scheres (@SjorsScheres) April 8, 2024
Cryo-electron microscopists need to understand this thread...if I hear one more time that the Shannon-Nyquist sampling theorem limits 3D reconstruction resolutions to double the camera pixel size I will be forced to commit seppuku in front of the classroom https://t.co/mfBHDEC7F4
— Jason Kaelber (@Kaelberviridae) April 2, 2024
Thoughts and opinions
"A structural biologist's dream" is the caption of this Figure 😉
— Eric Topol (@EricTopol) April 5, 2024
From a beautiful new review of cryo-EM for structural and cell biology @Nature https://t.co/hnHEdGEEnu pic.twitter.com/7tyinwyk5J
Biotech news and drug discovery technologies
'We beat big pharma': Biden applauds the Innovation Reduction Act, calls for a further reduction in R&D spending for new innovative medicines https://t.co/QhlNjcpLZO
— Bruce Booth (@LifeSciVC) April 4, 2024
Ozemphoria
Eli Lilly's edge over GLP-1 rivals tipped to drive Mounjaro sales to $34B by 2029 https://t.co/YRb2VMLmZr
— FiercePharma (@FiercePharma) April 17, 2024
GS:" The GLP-1 craze in pics $LLY $NVO...." pic.twitter.com/H4hFXG4P1J
— Tom Silver (@TomSilver39) April 15, 2024
Turns out that if tirzepatide is superior to semaglutide in a H2H study, the conclusions hold in a network meta-analysis #T2D @DiabetologiaJnl https://t.co/9deNeDevL6
— Daniel J Drucker (@DanielJDrucker) April 14, 2024
Oral Obesity Drugs Efficacy Comparison - @inversago's Monlunabant (CB1 inverse agonist) Ph1b (now Ph2) showed the steepest weight decline - yes still early pic.twitter.com/gV2CrDnoip
— Paras Sharma (@paras_biotech) April 13, 2024
About MiTeGen
MiTeGen, founded in 2004 by Cornell University Professor Robert Thorne, is a leading provider of innovative technologies for cryo-EM, crystallography, and other techniques used for probing small molecule and biomolecular structure and function. Our mission is to support researchers in achieving the best possible results throughout the entire process, from sample preparation to data collection.
With a strong emphasis on collaboration, we actively work with academic, government, and industrial researchers in over 40 countries to develop and deliver new tools and methods. Our products have gained recognition and trust within the scientific community, as they have been cited in over 4,500 published research articles. By leveraging concepts and technologies from physics and related fields, we tackle practical challenges faced by our friends and collaborators in the life sciences and other disciplines.
Driven by a passion for cryo cooling, we would like to showcase three of our innovative cryo-EM solutions:
Manual Plunge Cooler and Precision Ethane Cryostat:
Our Manual Plunge Cooler and Precision Cryostat are specifically designed to streamline and optimize your cryo-EM sample vitrification workflow. These systems consist of three components that can be obtained individually or as a complete package, ensuring efficient and successful sample preparation.
Cryo-EM Starter Kits:
Our Cryo-EM starter kits are curated to equip your laboratory with all the necessary tools conveniently packaged together. These kits are ideal for labs aiming to perform basic grid and sample preparation for cryo-EM within their own facility, even on a reduced budget. The kit includes components essential for cryo-EM sample preparation, as well as sample storage and transport solutions.
Cryo-EM AutoGrid Cassette Puck:
Our latest addition, the Cryo-EM AutoGrid Cassette Storage and Shipping puck, builds upon the success of our 2nd generation Cryo-EM Puck. This innovative solution allows users to screen samples on a instrument and seamlessly ship the same AutoGrid cassette puck to a collection facility. It also serves as a convenient storage option for facilities, enabling them to store their cassettes in the puck canes designed to fit into cassette loading stations.
These cryo-EM solutions exemplify our dedication to enhancing cryo cooling capabilities and simplifying workflows in the field of cryo-EM. MiTeGen continues to push the boundaries of innovation, enabling researchers to advance their structural biology studies with greater ease and efficiency.
Contact us at MiTeGen today to arrange a no obligation consultation to learn how we can help you with your research.
