Here are your MiTeGen Hottest Tweets in SBDD with exciting software developments such as newly...
MiTeGen Hottest Tweets in SBDD (07/23)
Here are your MiTeGen Hottest Tweets in SBDD, showcasing incredibly beautiful structures of targets, including an antigen-bound IgG3 complex, and the latest in cryo-EM related chatter:
Tough drug targets
IgG3 antibodies are the most potent IgG class, yet are the only class not used as a therapeutic! Partly as no one knew what they looked like. We solved the structure of antigen-bound IgG3 activating complement to uncover how these unique antibodies work:https://t.co/LEhefBO5nb pic.twitter.com/97GAsGCA8t
— Thom Sharp (@ThomSharp) July 7, 2023
How do deadly trypanosomes resist destruction by the complement system? Our study, led by @BiochemCook shows how their cell surface receptor, ISG65, binds to complement component C3, helping them evade killing. Now out in @eLife as a reviewed preprint!https://t.co/HCPREfQK2o pic.twitter.com/cA7S9QI9CY
— Matt Higgins (@ParasiteMatt) July 13, 2023
Our paper describing the 3Å #cryoEM structure and chain-elongating function of UBR5, one of the largest HECT E3 ligases, is now out! at https://t.co/dy9kMqKBqj pic.twitter.com/zQVEasq1Y1
— Zuzana Hodakova (@ZHodakova) July 6, 2023
Our latest paper exploring how MYC-MAX and CLOCK-BMAL1 engage with chromatinized E-boxes is out @Nature: https://t.co/fAaTOnaUp6. We thank all the collaborators for their valuable contributions! @jsmenet @partchlab @Beatfierz @SchubelerLab @johannes_zuber, Becker lab & Liu lab pic.twitter.com/EmuPodkLXG
— Thomä Lab (@LabThoma) July 6, 2023
No fiducials neededhttps://t.co/lW3p3nOdVR
— Nicholas Wright (@nick_rite) July 3, 2023
Happy to share structure of human TRPV4 in complex with the GTPase RhoA. TRPV4-RhoA contact interface reveals residues that are mutated in neuropathies, providing insight into disease pathogenesis. Great collaboration with the lab of David Zhang (MCW). https://t.co/1dgZh6U3RQ
— Alexander Sobolevsky (@AlexanderSobol6) June 23, 2023
We determined the structures of human UCP1 in apo, ATP-bound, and DNP-bound states. (https://t.co/D5BavNmElI) Congratulations to Yunlu on another two-author paper! pic.twitter.com/Yx1YTJ2RHp
— Lab of Lei Chen@PKU (@Chen_Lei_Lab) June 19, 2023
The weird and wonderful
Why is there so much disorder in eukaryotic proteins? What is its relationship with condensates? Why can some proteins be saved but others get degraded? After years in the making, we think we have answers and they're all connected. Excited to share a 🧵:https://t.co/gTFY7gAe4C
— Stephen Fried JHU (@fried_lab) June 26, 2023
Sample preparation
Our Manual Plunge Cooler and Precision Ethane Cryostat system for Cryo-EM are now available - Designed to make your cryo-EM sample vitrification workflow efficient and successful - Learn more https://t.co/baYrXczRVr Video https://t.co/AwUySNXaLG #cryoem pic.twitter.com/qLJIVMd1SD
— MiTeGen (@MiTeGen) June 30, 2023
Better imaging
When a student asks about number of turns of copper coil in an EM lens and you don't know…. CM120: 588. Thanks to our #workshop @MPIbp ! @thermosciEMSpec #cryoEM pic.twitter.com/PFajE0MYV5
— Sonja Welsch (@sonjawelsch) July 14, 2023
We suffered two problems on our Krios/Arctica instruments related to a pressure gauge called PPal. Here a picture of the faulty PPal from the Arctica. But then...what is a PPal and how it measures pressure? These two questions kept me busy for a while, a thread to follow 👇 pic.twitter.com/GsY95HNBxS
— Israel Fernandez (@IsraelF96135088) June 25, 2023
9yrs in and my neg stain TMV grid still does its mag cal work.
— Dr Cryo Mariena (@cryomariena) June 20, 2023
Processing and IT
I'm happy to present you the final version of Matteo's work on predicting functionally important sites in proteins using a machine-learning model that leverages structure and sequences and is trained using 2D-multiplexed data on abundance and activityhttps://t.co/YoIjLpRVIi pic.twitter.com/IbIHFoBi7O
— Kresten Lindorff-Larsen (@LindorffLarsen) July 13, 2023
#EMPIAR10 celebrates 10 years of releases! Contribution by @RadoDanev from the University of Tokyo.https://t.co/dpEP8nQuf4#CryoEM #CryoET #VolumeEM #XRM #SBFSEM #FIBSEM #MicroED #DeepLearning #BigData #OpenScience pic.twitter.com/Yeorpj572B
— EMDB - EMPIAR @EBI (@EMDB_EMPIAR) July 7, 2023
100-Billion param protein language model trained on ColabFoldDB 🤯
— Sergey Ovchinnikov 🇺🇦 (@sokrypton) July 7, 2023
Doing better than E15B (ESM2 15 billion) on structure prediction. https://t.co/gGNlKxnDcU pic.twitter.com/bJJT3a2nQD
Online now! GPCRana: A web server for quantitative analysis of GPCR structures https://t.co/VIzlemuvbb
— Structure (@Structure_CP) June 30, 2023
Thoughts and opinions
With todays easy-to-use processing tools, and courses focusing on more advanced processing, the basics often are forgotten. This is my new favorite lecture on Fourier transforms in cryo-EM! https://t.co/vQf287qXRb
— Wim Hagen (@WimJHH) July 11, 2023
That must have taken some courage, to speak up like that, for the early-career female scientist! 👏👏 https://t.co/5pLb4xEJzc
— Sjors Scheres (@SjorsScheres) July 1, 2023
And yet, again, apparently some scientists use AlphaFold as an easy way of downgrading structural biology grant proposals and in the intense competition often just one black mark / unthought through comment can push a proposal below the line.
— Ewan Birney (@ewanbirney) April 4, 2023
It has been a privilege and a dream for me to work at the LMB but truth is also that the discrepancy in salary between industry and academia is particularly bad in the UK and inflation is still sky high. I support "follow your dreams" but can see why not everyone can afford it.
— Andreas Boland (@BolandLab_GE) June 21, 2023
I am starting to get troubled by the use of deep learning software used to improve maps by the #cryoEM #SPA community. In the last #GRC2023, we saw maps that suffer from pathologies, such as preferential orientation becoming interpretable after treating them.
— Guillermo Montoya (@g1_guillermo) June 23, 2023
1)
in academia you get glory compared to industry. We are part of large teams and the glory is shared usually amongst many. In academia you get to learn about things that nobody knows yet, and divulge it, while in industry often we cannot share. I wouldn't say that we lack curiosity
— Chiara Rapisarda (@chiara_rapi) June 21, 2023
Biotech news and drug discovery technologies
Today, we announced the opening of our 70K sq ft CryoEM lab in Andover, Mass – among the largest privately owned labs of its kind in the U.S. – which will help drive faster and more efficient therapeutic development. Take an up-close look at the space:https://t.co/k6G0TiefjY pic.twitter.com/1VaTPlOuXS
— Generate Biomedicines (@generate_biomed) June 26, 2023
Past 3 biotech rallies & sales by the drugs
— Paras Sharma (@paras_biotech) June 25, 2023
1) Avastin (2005s)
2) Humira (2010s)
3) Keytruda (2015s)
4) Mounjaro/Ozempic (2020s) - has failed to translate into a rise in the overall bio index, with a re-rating carried out only for directly-benefiting companies: Lily & Novo pic.twitter.com/TLUdFdVzxu
A good read in Drug Discovery Today by colleagues @AstraZeneca: how is the changing landscape of targets (less ligandable, higher fraction of disordered regions) impacting approaches to identify small molecule hits. A lot is changing, for the better! https://t.co/o7dn0HY3VW pic.twitter.com/R6Ev9u9DLT
— Ingo Hartung (@HartungIngo) June 25, 2023
Scientist Stories: Andrew Kruse, GPCRs & Antibody Discoveryhttps://t.co/AyvWE21WbZ
— Cheers (@raintank2010) June 23, 2023
First oral semaglutide made a strong impression in OASIS-1 #obesity with 15% #weightloss now orforglipron leads the impressive pack of new small molecule oral GLP-1RA with robust #Weightloss and good tolerability ADA2023 pic.twitter.com/z3rAKXpLe5
— Daniel J Drucker (@DanielJDrucker) June 23, 2023
The glucagon-GLP-1 co-agonist class stands up tall #ADA2023 with impressive double digit #weightloss for survodutide @Boehringer @ZealandPharma #weightloss #obesity #NASH trials underway https://t.co/L8JdL0emUU
— Daniel J Drucker (@DanielJDrucker) June 23, 2023
Lilly & Novo combined added $140B of EV this year alone (the GLP-1 craze)
— Paras Sharma (@paras_biotech) June 19, 2023
However, its still a far cry from Apple's +$822B, Nvidia's +$694B & Tesla's +435B
From Torreya/Stifel pic.twitter.com/snc4iNqz1c
About MiTeGen
MiTeGen, founded in 2004 by Cornell University Professor Robert Thorne, is a leading provider of innovative technologies for cryo-EM, crystallography, and other techniques used for probing small molecule and biomolecular structure and function. Our mission is to support researchers in achieving the best possible results throughout the entire process, from sample preparation to data collection.
With a strong emphasis on collaboration, we actively work with academic, government, and industrial researchers in over 40 countries to develop and deliver new tools and methods. Our products have gained recognition and trust within the scientific community, as they have been cited in over 4,500 published research articles. By leveraging concepts and technologies from physics and related fields, we tackle practical challenges faced by our friends and collaborators in the life sciences and other disciplines.
Driven by a passion for cryo cooling, we would like to showcase three of our innovative cryo-EM solutions:
Manual Plunge Cooler and Precision Ethane Cryostat:
Our Manual Plunge Cooler and Precision Cryostat are specifically designed to streamline and optimize your cryo-EM sample vitrification workflow. These systems consist of three components that can be obtained individually or as a complete package, ensuring efficient and successful sample preparation.
Cryo-EM Starter Kits:
Our Cryo-EM starter kits are curated to equip your laboratory with all the necessary tools conveniently packaged together. These kits are ideal for labs aiming to perform basic grid and sample preparation for cryo-EM within their own facility, even on a reduced budget. The kit includes components essential for cryo-EM sample preparation, as well as sample storage and transport solutions.
Cryo-EM AutoGrid Cassette Puck:
Our latest addition, the Cryo-EM AutoGrid Cassette Storage and Shipping puck, builds upon the success of our 2nd generation Cryo-EM Puck. This innovative solution allows users to screen samples on a instrument and seamlessly ship the same AutoGrid cassette puck to a collection facility. It also serves as a convenient storage option for facilities, enabling them to store their cassettes in the puck canes designed to fit into cassette loading stations.
These cryo-EM solutions exemplify our dedication to enhancing cryo cooling capabilities and simplifying workflows in the field of cryo-EM. MiTeGen continues to push the boundaries of innovation, enabling researchers to advance their structural biology studies with greater ease and efficiency.
Contact us at MiTeGen today to arrange a no obligation consultation to learn how we can help you with your research.
