Here are your MiTeGen Hottest Tweets in SBDD, featuring heated discussions about the Inflation...
MiTeGen Hottest Tweets in SBDD (09/23)
Here are your MiTeGen Hottest Tweets in SBDD with DARPin scaffolds stealing the show cracking tough targets like LRRK2 and KRAS:
Setting up a new lab or know someone who is? Then contact us for special pricing & bundles on lab equipment and supplies to get the most out of those precious start-up funds. Let us be your partner! https://t.co/6CK4XKNI18 #crystallography #cryoEM #beamline #structuralbiology pic.twitter.com/DmSyL0ikdp
— MiTeGen (@MiTeGen) September 9, 2021
Tough drug targets
We report the first #cryoEM structures of #LRRK2 bound to type-1 and type-2 kinase inhibitors. Great team effort to advance Parkinson's Disease therapeutics research! #StefanKnapp @ChasingKinases @Marta_Sanz3 @acvillagransu @DedererVerena @ASAP_Research https://t.co/KrKzPbzRVb pic.twitter.com/AG92TVe1d9
— Andres Leschziner (@aleschziner) September 10, 2023
I'm thrilled to share our latest work on designing imaging scaffolds to determine the structure of small proteins using cryo-EM!https://t.co/FbXdjnABxG pic.twitter.com/gW9ThGFbjU
— Roger Castells (@rcastellsg) September 9, 2023
As promised, I've got our #IMC20 poster ready to go! If you're at the conference, swing by and say hi.
— Han Kim (@Kim_Hanseong) September 9, 2023
Got questions? Feel free to ask! pic.twitter.com/GYhvGeWS7K
Wow - xanomeline can be an orthosteric and allosteric agonist at the same time. Amazing how many times structures of GPCRs tell us something new about the pharmacology of ligands. Congratulations to the team at Monash for this fascinating work. https://t.co/q4yULGUqWa
— Fiona Marshall (@FionaHMarshall) September 7, 2023
Binding of antiepileptic drug Perampanel to complexes of AMPA receptor with auxiliary subunits y5 and CNIH2. Collaboration with Aryeh Korman and Drew Jones (NYU).@shantigangwar @LauraYen42 @MariaKarela3 https://t.co/TT7NaUOBcQ pic.twitter.com/JB7hTUjJeS
— Alexander Sobolevsky (@AlexanderSobol6) September 6, 2023
Congrats 🥳🥳🥳🥳🥳 https://t.co/xj1F7tUABH
— TKato (@oxkawaka) September 6, 2023
🚨Paper alert🚨
— Andreas Boland (@BolandLab_GE) September 1, 2023
Our new work on APC/C regulation revealed by high resolution cryo-EM structures together with the groups of Leifu Chang and David Barford.
Congratulations to Anna Höfler, Jun Yu and all other authors.
The manuscript can be found here:https://t.co/k5HGqDyfY5 pic.twitter.com/0ChhOSocNP
Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamicshttps://t.co/PMa3WSdDEs
— Cheers (@raintank2010) September 1, 2023
1/ We're excited to share our work on HIV-1 trafficking by dynein! Led by the amazing @badieyan in the lab with wonderful collaborations with @EdcampbellLab, @GiessenLab, Aiken Lab, Böcking Lab, and @morgan_desantis. TL;DR HIV-1 hijacks the dynein motor protein for motility 🧵 https://t.co/vwdTpgCCuk
— Michael Cianfrocco (@mcianfro) August 30, 2023
The weird and wonderful
Welcome to the future of structural biology. @AbhayKot @thermofisher in collab with @bengeliscious @FlorentWaltz unveil 5 mito complex structures in-situ from a single sample. And identify a potentially groundbreaking new membrane complex🔬🤩. Congrats everyone who contributed! pic.twitter.com/ElOKfgN4tD
— Alexey Amunts (@A_Amunts) September 18, 2023
Collaborated with @luningliu, we @NiLab_HKUMed synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. @OxfordStrubi @eBIC_Diamond https://t.co/GTtvJmmyl3
— the Peijun Zhang Lab (@GroupZhang) September 7, 2023
We started this project almost 10 years ago! Finally we managed to determine the #cryoEM structure of Makes Caterpillars Floppy I #toxin and elucidated its activation mechanism. Kudos to @alexanderbelyy1
— Stefan Raunser (@Intein) August 15, 2023
& @HeilenPhilipp and twitterless Philine & Oliver.
https://t.co/lvzopszF6c pic.twitter.com/WF41zZi04v
Sample preparation
When all modern kit for #cryoEM sample preparation (Leica, VitroBot, VitroJet) is out of order at the same time, it's time to dust off the classics 😎 pic.twitter.com/RZyzcXk8t6
— Juha Huiskonen (@JuhaHuiskonen) September 8, 2023
Cool! #cryoET of human brain tissue that was not previously frozen, representing tackling an important technical hurdle to studying #Alzheimer's disease in situ. #teamtomohttps://t.co/PIdCTbazOR
— Sjors Scheres (@SjorsScheres) September 18, 2023
While at EMBL, Felix and I have been working with Puxano for a few years now. Highly recommend trying their grids. If open science was truly open, they'd be dominating the grid supply market... https://t.co/tkKjiFlqxv
— Wim Hagen (@WimJHH) September 5, 2023
Pondering safe and efficient shipping of delicate, cryogenically frozen samples such as proteins or cryo-EM grids - Dry Shippers vs Dry Ice - https://t.co/93Oe3nML3L #cryoem #crystallography #structuralbiology pic.twitter.com/bOJLSgvU3M
— MiTeGen (@MiTeGen) September 18, 2023
Better imaging
CryoEM tweeps, how do you typically calibrate pixel sizes using e.g. apof data? Compare to existing structure? Use Cs refinement from processing? LocScale with —refine_apix argument?
— Wim Hagen (@WimJHH) September 17, 2023
Groundbreaking study from @mimi1inh and all members of @cmholab combining in situ #cryoET and #proteomics to determine the antimalarial activity of inhibiting translation in #cryoFIB-milled #malaria parasites:https://t.co/gBw6TNAdKP#cryoEM #massspec #teamtomo #modeofaction pic.twitter.com/z4IG3VxmG2
— Fitzpatrick Laboratory (@FitzpatrickLab) September 7, 2023
Want to set up #cryoEM in your lab? Learn all about it iyn this beginners course! 🥰 @Astbury_BSL is also a great place for doing structures of #amyloids. https://t.co/Ufr3cf0awn
— Sjors Scheres (@SjorsScheres) September 6, 2023
Processing and IT
Facing an #alphafold issue where the program detects homology with insanely large proteins (think Titin or similar) and crashes. How do I best fix this? Is there an exclude sequences > X aa flag or similar?
— Basil Greber (@BJ_Greber) September 13, 2023
Thoughts and opinions
Yes and we will never have a device like the iphone, or AI, or electric cars, or MRI machines or be able to make old cells young. We should give up. So complex.
— Robert Nelsen (@rtnarch) September 16, 2023
X is now basically 4chan with a better UI
— Yutong Zhao (@proteneer) September 10, 2023
Biotech news and drug discovery technologies
New online data and tool resources for basic research and #drugdesign on #GPCR and signaling proteins funded by Infrastructure grant from @novonordiskfond. Keep an eye out for GPCRdb, GproteinDb, ArrestinDb and BiasedSignalingAtlas and new databases. #DataScience @DrGPCR pic.twitter.com/5bP8jLxxS4
— GPCRdb (@gpcrdb) September 18, 2023
Congrats to the team on the great work here sharing the discovery of a low-molecular weight human interleukin-1β antagonist that blocks cytokine binding with the IL-1R1 receptor, revealing a novel hIL-1β binding pocket & potential new therapeutic target. https://t.co/L6D8ozyPQD
— Fiona Marshall (@FionaHMarshall) September 14, 2023
Obesity is on fire with 49 clinical stage programs, from 34 companies across 25 different MOAs
— Paras Sharma (@paras_biotech) September 10, 2023
From Liisa Bayko at Evercore pic.twitter.com/JMH7vB1sCL
Editorial overview: Artificial intelligence (AI) methodology in structural biologyhttps://t.co/IRlHB45MrD
— Cheers (@raintank2010) September 4, 2023
🚨 Today, we're thrilled to share our study expanding the gene editing toolbox to enable virtually any kind of edit in gene transcripts at the order of thousands of bases. A thread 🧵👇(1/n)https://t.co/zA4EyAVHFQ
— Basem Al-Shayeb, Ph.D. 🧬 (@themicrobeguy) August 19, 2023
About MiTeGen
MiTeGen, founded in 2004 by Cornell University Professor Robert Thorne, is a leading provider of innovative technologies for cryo-EM, crystallography, and other techniques used for probing small molecule and biomolecular structure and function. Our mission is to support researchers in achieving the best possible results throughout the entire process, from sample preparation to data collection.
With a strong emphasis on collaboration, we actively work with academic, government, and industrial researchers in over 40 countries to develop and deliver new tools and methods. Our products have gained recognition and trust within the scientific community, as they have been cited in over 4,500 published research articles. By leveraging concepts and technologies from physics and related fields, we tackle practical challenges faced by our friends and collaborators in the life sciences and other disciplines.
Driven by a passion for cryo cooling, we would like to showcase three of our innovative cryo-EM solutions:
Manual Plunge Cooler and Precision Ethane Cryostat:
Our Manual Plunge Cooler and Precision Cryostat are specifically designed to streamline and optimize your cryo-EM sample vitrification workflow. These systems consist of three components that can be obtained individually or as a complete package, ensuring efficient and successful sample preparation.
Cryo-EM Starter Kits:
Our Cryo-EM starter kits are curated to equip your laboratory with all the necessary tools conveniently packaged together. These kits are ideal for labs aiming to perform basic grid and sample preparation for cryo-EM within their own facility, even on a reduced budget. The kit includes components essential for cryo-EM sample preparation, as well as sample storage and transport solutions.
Cryo-EM AutoGrid Cassette Puck:
Our latest addition, the Cryo-EM AutoGrid Cassette Storage and Shipping puck, builds upon the success of our 2nd generation Cryo-EM Puck. This innovative solution allows users to screen samples on a instrument and seamlessly ship the same AutoGrid cassette puck to a collection facility. It also serves as a convenient storage option for facilities, enabling them to store their cassettes in the puck canes designed to fit into cassette loading stations.
These cryo-EM solutions exemplify our dedication to enhancing cryo cooling capabilities and simplifying workflows in the field of cryo-EM. MiTeGen continues to push the boundaries of innovation, enabling researchers to advance their structural biology studies with greater ease and efficiency.
Contact us at MiTeGen today to arrange a no obligation consultation to learn how we can help you with your research.
