Here are your MiTeGen Hottest Tweets in SBDD with exciting software developments such as newly...
MiTeGen Hottest Tweets in SBDD (08/23)
In this edition of MiTeGen Hottest Tweets in SBDD, the biotech world is manic about incretin mimetics, while the cryo-EM community nostalgically longs for FSC resolution cutoffs. Also check out this cool Ethane Cryostat for ethane temperature control on your Vitrobot:
Our NEW Ethane Cryostat for the MiTeGen Manual Plunge Cooler https://t.co/CFRKMvKKbX or Vitrobot: temp-controlled liquid ethane for user-friendly vitrification, reducing risks to your samples and equipment - Learn More https://t.co/4gtksZ6beX #cryoem pic.twitter.com/ipGYVMvsPo
— MiTeGen (@MiTeGen) July 25, 2023
Tough drug targets
Our new @ACSBioMed JMedChem is out in ASAPs describing the discovery of our #PfizerChemistry small molecule orally-active GLP1 agonist. Thank you to a great team from so many disciplines that made this possiblehttps://t.co/5JEi2ZTuvk
— Scott Bagley (@BagPhos) June 1, 2022
Tail engagement of arrestin at the glucagon receptorhttps://t.co/JtVFiTiDvM
— Cheers (@raintank2010) August 10, 2023
new release2
— Hideaki E. Kato (@emeKato) August 9, 2023
8JPB: NTSR1-GRK2-Gαq complexes 1
8JPC: NTSR1-GRK2-Gαq complexes 2
Focused refinement structure of
8JPD: GRK2
8JPE: Gαq
8JPF: NTSR1
in NTSR1-GRK2-Gαq complexes
"GPCR activation and GRK2 assembly by a biased intracellular agonist."
Xu lab. Nature. pic.twitter.com/4rSEUdHbvI
Here, high-resolution #cryoEM structures help pave the way to facilitate rational drug design of novel and optimized Prostaglandin F2α Receptor-targeting drugs. Congrats to the Chinese Academy of Sciences Shanghai for these fantastic results! https://t.co/3SYXjuXygq
— Ieva Drulyte (@IevaDrulyte) August 9, 2023
Mitochondrial stress causes DELE1 to form a large signaling scaffold that activates the integrated stress response. Congrats @_JieYang for initiating this project several years ago, and our amazing collaborators in the @LabWiseman and @KampmannLab labs! https://t.co/pqzZrB7dKz pic.twitter.com/d0HHrzBN03
— Lander Lab (@LanderLab) August 7, 2023
Mitochondrial stress causes DELE1 to form a large signaling scaffold that activates the integrated stress response. Congrats @_JieYang for initiating this project several years ago, and our amazing collaborators in the @LabWiseman and @KampmannLab labs! https://t.co/pqzZrB7dKz pic.twitter.com/d0HHrzBN03
— Lander Lab (@LanderLab) August 7, 2023
A cancer vaccine is delivered to the lung by an engineered attenuated influenza virus https://t.co/Wm1UW9S2xe
— Nature Biotechnology (@NatureBiotech) August 8, 2023
Targeted lipid modification may provide a single intervention strategy for design of long-acting, nonselective, Amylin-based DACRAs with potential anti- #obesity effects @nchembio https://t.co/lodxvRLa7I
— Daniel J Drucker (@DanielJDrucker) August 4, 2023
Not super sure when is best to tweet in the new @eLife process, and I probably missed the boat 😅, but out today is the "version of record" for our work on the regulation of membrane receptor guanylyl cyclases. (1/3) https://t.co/1fjVkk3FFy pic.twitter.com/hxHCI8IzF2
— Nathanael Caveney (@CaveneyNA) August 3, 2023
#CryoEM structures by @DianaArseni in @bwfalcon's group reveal that TDP-43 forms amyloid filaments with a new fold in the most common type of frontotemporal lobar degeneration: https://t.co/MAJSlt9SMo#LMBResearch pic.twitter.com/77AhH84vlL
— MRC Laboratory of Molecular Biology (@MRC_LMB) August 2, 2023
new release2
— Hideaki E. Kato (@emeKato) August 2, 2023
8JR9: PCO371 bound to human PTH1R
"A Cryo_EM structure of class B G protein coupled receptors for 2 orally active small molecule agonists"
→"Conserved class B GPCR activation by a biased intracellular agonist"
Eric Xu lab. Nature. pic.twitter.com/oj5DegCbF3
new release1
— Hideaki E. Kato (@emeKato) August 2, 2023
8F0J: CTR w/ Gs and Pramlintide analogue peptide San45
AMY3 Receptor w/ Gs and
8F0K: San385
8F2A: San385 (Cluster 5 conf)
8F2B: San45
"Structure-based insight into development of selective and non-selective amylin and calcitonin receptor agonists"
Wootten lab. pic.twitter.com/1TBUF4e6Z9
Disease-specific tau filaments assemble via polymorphic intermediates. This is what had me so excited for the past few weeks! The amazing Sofia Lövestam has solved another 163 #cryoEM structures! 🥳🥳🥳A #tweetorial below. As always, with @MichelGoed.https://t.co/bQTM8gN9sO
— Sjors Scheres (@SjorsScheres) July 27, 2023
Excited to share the structure of an orphan #GPCR GPR179 bound to an extracellular cell adhesion molecule Pikachurin (EGFLAM) which is key to light reception. Great collaboration with @fornerislab, @AppuKSingh_IITK and Pat Griffin. https://t.co/CGm0AvdD9r pic.twitter.com/UDo09iEzr1
— Kirill Martemyanov Lab (@KLabGPCR) July 25, 2023
The weird and wonderful
Happy to share our work on the structure of the human outer kinetochore KMN network complex
— Stanislau Yatskevich (@StanislauY) August 8, 2023
Many thanks to David Barford, Dom Bellini, @JYang188 and @ZhangZiguo for working with me on this project.https://t.co/BQuK5cmwKc pic.twitter.com/v8E78o53LX
Sample preparation
Shameless plug. Here's a new protocol paper for EMPEM. 😊https://t.co/lss3jeTTNK
— EMproblems (@CryoEMprobs) August 1, 2023
https://t.co/9FLfRdZfw0
— George Vaisey (@GVaisey) April 25, 2023
Next generation of membrane protein single particle cryo-EM is here. Amazing works from my colleagues @Chen_Zhao_CZhao and @XiaoTao214426. High resolution structure of Slo1 in near native conditions - no detergent at all!
Our NEW Ethane Cryostat for the MiTeGen Manual Plunge Cooler https://t.co/CFRKMvKKbX or Vitrobot: temp-controlled liquid ethane for user-friendly vitrification, reducing risks to your samples and equipment - Learn More https://t.co/4gtksZ6beX #cryoem pic.twitter.com/ipGYVMvsPo
— MiTeGen (@MiTeGen) July 25, 2023
When the @thermosciEMSpec #Glacios Autoloader vacuum sucks (or rather doesn't as it should), you inspect its valve to the outside world - and find this… Simple explanations are a wonderful thing. #cryoEM @MPIbp pic.twitter.com/3fiO7rUTda
— Sonja Welsch (@sonjawelsch) July 24, 2023
Better imaging
Radiolysis of Thin Water Ice in Electron Microscopy | The Journal of Physical Chemistry C https://t.co/CP7COjjULp
— Wim Hagen (@WimJHH) August 2, 2023
Square electrons! Get your square electron beams!
— Alex J Noble (@alexjamesnoble) July 31, 2023
Replace your C2 with a square aperture. Benefits include optimal tiling in cryoEM & cryoET and no resolution loss.
Paper: https://t.co/WsI3p8561l
Study by @eydchua, Michael Alink, @MishaKopylov3, & @demarco_lab, @SEMC_NYSBC https://t.co/RErlw8hdlF pic.twitter.com/TWAOV4sROn
Processing and IT
🔔Don't miss this opportunity and Join our #singleparticlecryoEM data processing workshop at #bio21 #IHIC on 23rd-25th of October. Register here:https://t.co/wG0VWWRUqg. Registration closes on September 15th.
— Sepideh Valimehr (@SValimehr) August 4, 2023
Any #cryoEM facility users/staff with a take on ideal balance of EM user guidance vs. freedom?🧐Users working through scope hiccups learn a lot, but create chaos. Those shielded from hiccups generate more data, but not fewer issues (just different). And never grow into experts.🤷🏻♀️ pic.twitter.com/34pkVPr3cc
— Sonja Welsch (@sonjawelsch) July 28, 2023
Thoughts and opinions
Henderson and Hasnain: `Cryo-EM': electron cryomicroscopy, cryo electron microscopy or something else? #Nomenclature #CryoEM #Standardization @livuni... #IUCr https://t.co/JCvvh0s5Lf
— IUCrJ (@IUCrJ) August 11, 2023
When you read posts like this, it's clear that the biopharma industry has done a terrible job communicating the value prop of our industry.
— Andrew Pannu (@andrewpannu) August 13, 2023
A reminder on some of those points:
Grab a coffee, this will be a long one
✔️ Drug spending is far less than hospital care & physician… https://t.co/CZfYhBVTcp pic.twitter.com/nn4MzWQVbL
Can cryoEM go back to the more entertaining topics? 2/3 Nyquist, FSC, near-atomic, p-length in 1/Å?
— Wim Hagen (@WimJHH) August 12, 2023
This!
— Robert Insall @robinsall@mas.to (@robinsall) August 9, 2023
Proteins are there as part of a huge, stochastic system. Not to fulfil a purpose.
A cell is not a car. You can't look at one doohicky and say "that's there in order to pump fuel". Because nobody designed it, and proteins aren't engineered for a unique function. #evolution https://t.co/OBogR4CNzt
When I started out in science, I ran gels. It takes a day or two to master and then you just do it hundreds of times. If you fail, it costs your time only & you try again.
— Jason Kaelber (@Kaelberviridae) July 31, 2023
CryoEM takes months to master and a typical PhD student will solve <5 structures. How do we train?
Biotech news and drug discovery technologies
This week on the Readout LOUD:
— Adam Feuerstein (@adamfeuerstein) August 10, 2023
Wegovy! Of course.
Can $LLY become the first trillion-dollar drug maker?
We discuss w/ Jared Holz, biotech market strategist at Mizuho. https://t.co/B2yIMPpOU8
Meanwhile, over at Indianapolis @EliLillyandCo Tirzepatide is driving tremendous sales growth #T2D and innovative oral GLP-1 Orforglipron and triple G Retatrutide are now in P3 and poised to move the needle in promising new options for #T2D #obesity #CVD https://t.co/PQofrSnkVa pic.twitter.com/sSw5DEC1dx
— Daniel J Drucker (@DanielJDrucker) August 8, 2023
Wow, wow, wow!
— Sek Kathiresan MD (@skathire) August 8, 2023
In SELECT trial of patients with obesity (mean BMI 33, n>17,000) & prior atherosclerotic cardiovascular disease (but no diabetes),
GLP-1 agonist semaglutide 2.4mg once-weekly
reduced risk of CV death, heart attack, or stroke (RRR 20%)https://t.co/BQvWUujknh pic.twitter.com/aotdqUKs2K
The @US_FDA just approved the first oral medication indicated to treat postpartum depression in adults. https://t.co/jDNFI2HktH https://t.co/YQ9PIiez8x
— National Institute of Mental Health (NIMH) (@NIMHgov) August 7, 2023
On GLP-1s & the new weight loss drugs, Pfizer CEO said: "this is only the beginning of this space. Clearly the unmet medical need is huge--obesity. Lilly & Novo Nordisk are here very successfully with two products that are injectable. There is a lot of market research conducted… https://t.co/fBzUvt0RS5
— Pearl Freier (@PearlF) August 1, 2023
Apparently this phenomenon has a name: Pipeline herding. What a waste of time and money, especially in oncology https://t.co/5Z9USkPp1V https://t.co/ivHjbMl1w1 pic.twitter.com/EH1B8ePfHt
— Ohad Hammer (@ohadhammer) July 31, 2023
Some discouraging stats from a new analysis of big pharma R&D productivity: "On average, a big pharma company spent $4.4 billion annually in R&D and launched 0.78 new drugs. The peer group's R&D efficiency (2001–2020) is $6.16 billion (total R&D spending per new drug)" pic.twitter.com/QL0xEArTtP
— Alex Telford (@Atelfo) July 31, 2023
Mirror mirror on the IP wall- which GLP-1- based medicines have the strongest patent protection of them all? https://t.co/Ur2v3KrKbz
— Daniel J Drucker (@DanielJDrucker) July 28, 2023
About MiTeGen
MiTeGen, founded in 2004 by Cornell University Professor Robert Thorne, is a leading provider of innovative technologies for cryo-EM, crystallography, and other techniques used for probing small molecule and biomolecular structure and function. Our mission is to support researchers in achieving the best possible results throughout the entire process, from sample preparation to data collection.
With a strong emphasis on collaboration, we actively work with academic, government, and industrial researchers in over 40 countries to develop and deliver new tools and methods. Our products have gained recognition and trust within the scientific community, as they have been cited in over 4,500 published research articles. By leveraging concepts and technologies from physics and related fields, we tackle practical challenges faced by our friends and collaborators in the life sciences and other disciplines.
Driven by a passion for cryo cooling, we would like to showcase three of our innovative cryo-EM solutions:
Manual Plunge Cooler and Precision Ethane Cryostat:
Our Manual Plunge Cooler and Precision Cryostat are specifically designed to streamline and optimize your cryo-EM sample vitrification workflow. These systems consist of three components that can be obtained individually or as a complete package, ensuring efficient and successful sample preparation.
Cryo-EM Starter Kits:
Our Cryo-EM starter kits are curated to equip your laboratory with all the necessary tools conveniently packaged together. These kits are ideal for labs aiming to perform basic grid and sample preparation for cryo-EM within their own facility, even on a reduced budget. The kit includes components essential for cryo-EM sample preparation, as well as sample storage and transport solutions.
Cryo-EM AutoGrid Cassette Puck:
Our latest addition, the Cryo-EM AutoGrid Cassette Storage and Shipping puck, builds upon the success of our 2nd generation Cryo-EM Puck. This innovative solution allows users to screen samples on a instrument and seamlessly ship the same AutoGrid cassette puck to a collection facility. It also serves as a convenient storage option for facilities, enabling them to store their cassettes in the puck canes designed to fit into cassette loading stations.
These cryo-EM solutions exemplify our dedication to enhancing cryo cooling capabilities and simplifying workflows in the field of cryo-EM. MiTeGen continues to push the boundaries of innovation, enabling researchers to advance their structural biology studies with greater ease and efficiency.
Contact us at MiTeGen today to arrange a no obligation consultation to learn how we can help you with your research.
